RBRC00445 Masaru OKABE Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> 岡部　勝 C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 237, 222-231 (2001).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. Transgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG). 開発者：岡部勝先生機関名：大阪大学遺伝子情報実験センター Carrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc]. Fluorescent Proteins/lacZ System Osaka Univ. C (3-6 months) 480 nmで励起すれば、体および精子の先体が光る。 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Biol., 237, 222-231 (2001).<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること（https://www.ccb.osaka-u.ac.jp/en/）。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 大阪大学 開発者：岡部勝先生 機関名：大阪大学遺伝子情報実験センター C57BL/6-TgN(acro/act-EGFP)OsbC3-N01-FJ002 C57BL/6-TgN(acro/act-EGFP)OsbC3-N01-FJ002 C（3〜6か月） Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The CAG and acr-EGFP plasmids were co-injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6NCr background. <a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/00445_GB.pdf">Genetic Background</a> CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, rabbit beta-globin polyadenylation signal true <a href='https://brc.riken.jp/mus/pcr00445'>Genotyping protocol -PCR-</a> 特になし。+N10?+F3。 トランスジーンはホモ型に固定されている。兄妹交配による維持。 Reproductive Biology Research